Antifungal activity of honey bee Plebeia cf. flavocincta against Aspergillus niger

Authors

  • Ana Paula Vitorino da Silva Universidade Federal de Campina Grande, Pombal
  • Maria Carmem Batista de Alencar Universidade Federal de Campina Grande, Pombal
  • Patricio Borges Maracajá Universidade Federal de Campina Grande, Pombal
  • Symara Abrantes A. de O. Cabral Universidade Federal de Campina Grande, Pombal
  • Daniel Casimiro da Silveira Universidade Federal de Campina Grande, Pombal
  • Egberto Santos Carmo Universidade Federal de Campina Grande, Pombal

DOI:

https://doi.org/10.18378/aab.v3i1.3500

Keywords:

Honey, Antimicrobial, Plebeia, Aspergillus niger

Abstract

The bee produces various products, among which honey is considered the most important because of its nutritional and medicinal value. In recent decades due the appearance of pathogens resistant to antibiotics and the incidence of collateral effects caused by medicines, scholars have investigated the antimicrobial properties of different natural products, such as honey. In Brazil there are few studies about the honey of the stingless bee Plebeia cf. Flavocincta and so this study aimed to verify the antifungal activity of the honey of this bee against Aspergillus niger come from human otitis. To that end, through the microdilution technique using microtitre plates containing 96 cavities with bottom of “U” was determined by Minimum Inhibitory Concentration (MIC) of that honey. Then was determined the Minimum Fungicidal Concentration (MFC) to lower concentrations where was not visualized the fungal growth. The whole system was incubated at 28 ° C for up to 5 days. The assays were performed in triplicate and the result is expressed by the geometric mean of MIC and MFC values obtained. Performed the analysis, we obtained a MIC equal to 50% and MFC equivalent to the pure honey. It is concluded that the honey of Plebeia cf. flavocincta bee was active against the fungus A. niger, but in high concentrations, interesting fact, because it is the way many people use some type of honey popularly for the treatment of otitis, while it is clear that dilutions of this product are not effective, especially against A. niger.

Downloads

Download data is not yet available.

References

ADAMS, C. J. et al.Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey. Carbohydrate Research. Hamilton: Elsevier, v. 343, p. 651–659, 2008.

AL-WAILI, N. S. Topical honey application vs. acyclovir for the treatment of recurrent herpes simplex lesions. Medical Science Monitor. [S.I.: s.n.], v. 10, p. 94 – 98, 2004.

ANDERSON, D., Antioxidant defenses against reactive oxigen species causing genetic and other damage. Mutation Research, Amsterdam, Netherlands, v. 350, n. 1, p. 103 - 108, 1996.

ATANDA, O. O.; AKPAN, I.; OLUWAFEMI, F.The potential of some essential oils in the control of A. parasiticus CFR 223 and aflatoxin production. Food Control. v. 18, n. 5, p. 601-607, 2007.

AVILA-PIRES, F. D. de. Teoria e prática das práticas alternativas. Revista de Saúde Pública. V. 29, n. 2, p. 147 – 151, 1995.

BARROS, M. E. S; SANTOS, D. A.; HAMDAN, J. S.In vitro methods for antifungal susceptibility testing of Trichophyton spp. Mycological Research. v. 110, n. 11, p. 1355-1360, 2006.

BAZONI, M. O. Atividade antimicrobiana dos méis produzidos por Apis melífera e abelhas sem ferrão nativas do Brasil. Ribeirão Preto: [s. i.], p. 116,. (Tese de Doutorado) – Faculdade de Medicina de Ribeirão Preto, São Paulo, 2012.

BESSOLI, E. D. G. Apostila de Patologia Clinica Médica e terapêutica de pequenos animais. [S.I.: s.n.], 2000.

BOBANY, D. de M. et al. Atividade antimicrobiana do mel de abelhas jataí (Tetragonisca angustula) em cultivos de microrganismos do conduto auditivo de caninos domésticos (Canis familiaris). Ci. Anim. Bras. Goiânia: [s.i.], v. 11, n.2, p. 441 – 446, 2010.

BRODY, T. M. et al. Farmacologia humana: da molecular a clínica. 2. ed. Rio de Janeiro: Guanabara Koogan, p. 457 - 637, 1994.

BROWN, K. D.; BANUCHJ, V.; SELESNICK, S. H. Doença da orelha externa. In: LALWANI, A. K. Current Otorrinolaringologia Cirurgia de cabeça e pescoço: Diagnóstico e Tratamento. Tradução por Ademar V. F.; Maria da G. F. da S. T.; Rita B. da S. P. Porto Alegre: AMGH Editora LTDA, 3 ed., c. 47, p. 643 - 648, 2013.

CANUTO, M. M.; RODERO, F. G. Antifungal drug resistance to azoles and polyenes. The Lancet Infectious Diseases. v. 2, n. 9, p. 550 - 563, 2002.

CORTOPASSI-LAURINO, M.; GELLI, D. S. Analyse pollinique, propriétés physico-chimiques et action antibactérienne des mieis d’abellies africanisé es Apis mellifera et de Méliponinés Du Brésil. Apidologie, v. 22, p. 61 - 73, 1991.

CURTIS, L. et al. Aspergillus surveillance project at a large tertiary-care hospital. Journal of Hospital Infection. v. 59, n. 3, p. 188 - 196, 2005.

D’ARCY, B. R. Antioxidants in Australian Floral Honeys–Identification of health-enhancing nutrient components. Rural Industries Research and Development Corporation. Brisbane, Australia: [s.n.], 94 p., 2005.

DESSELBERGER, V. Emerging and re-emerging infectious disease. Journal of Infectious Diseases. v. 40, n. 1, p. 3 - 15, 2000.

EMBRAPA. Produção de mel. Sistemas de produção 3. Piauí, Teresina: [s.n.], 138 p., 2002.

FREITAS, B. M. Meliponíneos. Fortaleza: [s.n.], p. 9, 2003.

FUCHS, F. D.; WANNMACHER, L.; FERREIRA, M. B. C. Farmacologia clinica. Fundamentos da terapêutica racional. 3. ed. Rio de Janeiro: Guanabara Koogan. p. 439 - 440, 2004.

FUKUDA, M. et al. Jungle honey enhances immune function and antitumor activity. Evidence-based Complementary and Alternative Medicine. [S.I.: s.n.], v. 10, p. 1 - 8, 2009.

GARCIA, M. A.; FERNANDEZ, M. I.; MELGAR, M. J. Contamination of honey with organophosphorus pesticides. Bull Environ ContamToxicol. [S.I.: s.n.], v. 54, p. 528–532, 1996.

GONÇALVES, A. L., ALVES FILHO, A., MENEZES, H. Atividade antimicrobiana do mel da abelha nativa sem ferrão Nannotrigona testaceicornis (Hymenoptera: Apidae, meliponini). Arquivo Instituto de Biologia. São Paulo: [s.n.], v. 72, n. 4, p. 455 - 459, 2005.

HADACEK, F.; GREGER, H. Testing of antifungical natural products: methodologies, comparability of results and assay choise. Phytochemical Analysis, v.11, p. 137-147, 2000.

HAMZAOGLU, I. et al. Protective covering of surgical wound with honey impedes tumor implantation. Archives of Surgery. [S.I.: s.n.], v. 135, p. 14–17, 2000.

HENRIQUES, A. Mel: um milagre da natureza para o tratamento de feridas? School of applied Sciences. University of Wales Institute; Wales-Cardiff, UK: [s.n.], 2004.

KERN, M. E.; BLEVINS, K. S. Micologia médica: texto e atlas. 2 ed. São Paulo: Premier, 256 p., 1999.

KONTOYIANNIS, D. P.; LEWIS, R. E. Antifungal drug resistance of pathogenic fungi. Lancet, v. 359, n. 9312, p. 1135-1144, 2002.

KWAKMAN, P. H. S. et al. How honey kills bactéria. The Journal of the Federation of American Societies for Experimental Biology. Amsterdam: [s.n.], v. 24, n. 7, p. 2576-2582, 2010.

LUZ, M. T. Cultura contemporânea e medicinas alternativas: novos paradigmas em saúde no Fim do Século XXI. Revista de Saúde Coletiva. V. 15, p. 145 – 176, 2005.

MAGALON, G.; VANDWIJCK, R. Guide des Plates: Du pansement à La chirurgie. John Libbey Eurotext. [S.I.: s.n.], p. 103, 2003.

MAVRIC, E. et al. Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand. Molecular Nutrition & Food Research, v. 52, p. 483 – 489, 2008.

MEDLEAU, L.; HNILICA, K. A. Dermatologia de Pequenos Animais: atlas colorido e guia terapêutico. 2 ed. São Paulo: Roca, 2003.

MOLAN, P. C. The antibacterial activity of honey: The nature of the antibacterial activity. Bee World, Hamilton, New Zealand: [s.n.], v. 73, n.1, p. 5 - 28, 1992a.

MOORE, C. B. et al. Antifungal drug resistance in Aspergillus. Journal of Infection. v. 41, n. 3, p. 203 - 220, 2000.

MOORE, O. A. et al. Systematic rewiew of the use of honey as a wound dressing. BMC Complementary and Alternative Medicine, [S.I.: s.n.], p. 2, 2001.

MOREIRA, A. C. P. et al. Chemical composition and antifungal activity of Hyptissuaveolens (L.) poit leaves essential oil against Aspergillus species. Brazilian Journal of Microbiology, v. 41, n. 1, p. 28 - 33, 2010.

MOUSSALE, S. K.; LUNA, R.; MARQUARDT, R. C. Otite externa fúngica. International archives of Otorhinolaryngology, v. 3, n. 4, 1999.

MUÑOZ O. et al. Contenido de flavonoides y compuestos fenólicos de mieles chilenas e índice antioxidante. Quim.Nova, v. 30, n. 4, p. 848 - 851, 2007.

NOGUEIRA, J. C. R. et al. Identificação e suscetibilidade antimicrobiana de microrganismos obtidos de otite externa aguda. Revista Brasileira de Otorrinoloralingologia. São Paulo: [s.n.], v. 74, n. 4, 2008.

OETTERER, M.; D’ARCE M. A. B. R.; SPOTO, M. Fundamentos de ciência e tecnologia de alimentos. Barueri, SP: Manole, 2006.

OSTROSKY, E. A. et al. Métodos para avaliação da atividade antimicrobiana e determinação da concentração mínima inibitória (CMI) de plantas medicinais. Revista Brasileira de Farmacognosia. [S.n.: s.i.], p. 301-307, 2008.

PAUL, I. M. et al. Effect of honey, dextromethorphan, and no treatment on nocturnal cough and sleep quality for coughing children and their parents.Archives of Pediatrics and Adolescent Medicine. [S.I.:s.n.], v. 161, p. 1140 – 1146, December, 2007.

POSEY, D. A. Etnoentomologia de tribos indígenas da Amazônia. In: RIBEIRO, D. Suma etnológica brasileira. 2.ed. Petrópolis: FINEP/Vozes, p. 251 - 271, 1987.

PFALLER, M. A. et al. Antifungal activity of posaconazole, ravuconazole e voriconazole compared to that of itraconazole tested against 213 clinical isolates of Aspergillus spp. and other filamentous fungi: report from the Sentry Antimicrobial Surveillance Program, 2000. In: Abstract of the 41st Interscience Conference on Antimicrobial Agents and Chemotherapy. Abstract J-813, p. 379, 2001.

RASOOLI, I.; ABYANEH, M. R. Inhibitory effects of thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control. v. 15, n. 6, p. 479 - 483, 2004.

ROSA, C. A. R.; CAMPOS, S. G.; BARONI, F. A. Micologia veterinária: prática 8. [2002]. 12 f. Notas de aula. Impresso.

SAHIN, F. et al. Biological activities of the essential oil and methanol extract of Origanum vulgares sp vulgare in the Eastern Anatolia region of Turkey. Food Control. v. 15, n. 7, p. 549 - 557, 2004.

SARTORATTO, A. et al. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology, v. 35, p. 275-280, 2004.

SEBRAE. Cosméticos à base de produtos naturais. Estudo de Mercado SEBRAE/ESPM. [S.I.: s.n.], p. 100, 2008.

SIDRIM, J. J. C.; MOREIRA, J. L. B. Fundamentos clinico-laboratoriais da micologia medica. Rio de Janeiro: Guanabara Koogan, p. 171 - 190, 1999.

SIDRIM, J. J. C.; ROCHA, M. F. G. Micologia médica à luz de autores conteporâneos. [Reimpr.] Rio de Janeiro: Guanabara Koogan, 2010.

SILVA, R. A. da et al. Composição e propriedades terapêuticas do mel de abelha. Alimentos e Nutrição, Araraquara, v. 17, n. 1, p. 113 – 120, 2006.

SILVA, T. M. S. et al. Phenolic compounds, melissopalynological, physicochemical analysis and antioxidant activity of jandaira ( Meliponasubnitida) honey. Journal of Food Composition and Analysis. [S.I.]: Elsevier, v. 14, p. 678-684, 2012.

SOARES, M. A. Medicamentos Não Prescritos. Aconselhamento Farmacêutico. 2 ed. Lisboa: Associação Nacional de Farmácias, v. II, 2002.

SOUZA, E. L. et al. Effectiveness of Origanum vulgare L. essential oil to inhibit the growth of food spoiling yeasts. Food Control, v. 18, n. 5, p. 409 – 413, 2007.

SWELLAM, T. et al. Antineoplastic activity of honey in an experimental bladder cancer implantation model: In vivo and in vitro studies. International Journal of Urology, v. 10, p. 213 - 219, 2003.

TILLEY, L. P.; SMITH, F. W. K. Consulta veterinária em 5 minutos. 2 ed. Barueri: Manole, 2008.

TRABULSI, L. R.; ALTERTHUM, F. Microbiologia. 5 ed. São Paulo: Atheneu, 2008.

TKACKZ, J. S.; DIDOMENICO, B. Antifungals: what’s in pipeline. Current Opinion in Microbiology, n. 4, p. 540 – 545, 2001.

UMVF – ANOFEL. Aspergilloses et autres champignons filamenteux opportunistes. 2014.

VIT, P.; TOMÁS-BARBERÁN, F. A. Flavonoids in meliponinae honeys from Venezuela related to their botanical, geographical and entomological origin to assess their putative anticataract activity. Zeitschrift für Lebensmittel- Untersuchung und – Forschung. v. 206, p. 288 - 293,1998.

WELCH, K. D. et al. Deleterious ironmediated oxidation of biomolecules. Free Radical Biology and Medicine, Logan, USA, v. 32, p. 577 - 583, 2002.

ZUMLA, A.; LULAT, A. Honey- a remedy rediscovered. Journal of the Royal Society of Medicine, v. 82, p. 384 - 385, 1989.

Published

2015-02-13

How to Cite

SILVA, A. P. V. da; ALENCAR, M. C. B. de; MARACAJÁ, P. B.; CABRAL, S. A. A. de O.; SILVEIRA, D. C. da; CARMO, E. S. Antifungal activity of honey bee Plebeia cf. flavocincta against Aspergillus niger. ACTA Apicola Brasilica, [S. l.], v. 3, n. 1, p. 01–09, 2015. DOI: 10.18378/aab.v3i1.3500. Disponível em: https://www.gvaa.com.br/revista/index.php/APB/article/view/3500. Acesso em: 28 mar. 2024.

Issue

Section

ARTICLES

Most read articles by the same author(s)