Testes de toxicidade de íons metais usando Paramecium caudatum como organismo teste
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Os experimentos de avaliação da toxicidade dos metais foram feitos com organismos obtidos na fase log de crescimento em meio de cultivo padronizado e previamente estabelecido, após obtenção de sua curva de crescimento e tempo de geração, seguindo recomendações do processo de otimização do cultivo para o protozoário Paramecium caudatum. Os testes foram realizados com dezoito íons cloreto de metais, frente ao protozoário em seis diferentes concentrações em meio aquoso. Os resultados dos testes de toxicidade mostraram que a tolerância desse protozoário é maior ao molibdênio e ao lítio (CL50 24 h. > 4,5 mg.L-1 e 3,69 mg.L-1; respectivamente), enquanto que para o mercúrio e o cobre foram iguais e as menores (CL50 24 h. ≥ 0,021 mg.L-1). A ordem decrescente de toxicidade dos metais testados nos ensaios com Paramecium caudatum foi a seguinte: Hg > Cd > As > Mn > Pb > Cr . Comparando as concentrações máximas permitidas desses metais pela legislação brasileira, com os resultados de tolerância obtidos nos experimentos com esse protozoário, nota-se que Paramecium caudatum é sensível aos mesmos em concentrações inferiores às máximas permitidas a esses metais, evidenciando a importância de se realizar ensaios com esses organismos na avaliação de contaminações, especialmente por metais; e no monitoramento ambiental.
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ALBA, A.R.F, GUIL, L.H., LÓPEZ, G.D. AND CHISTI, Y. Toxicity of pesticides in wastewater: a comparative assessment of rapid bioassays. Analytica Chemycal Acta, 426 (2):289-301, (2001);
ALBERGONI V, PICCINNI E. Biological response to trace metals and their biochemical effects. In: Leppard GG (ed) Trace element speciation in surface waters and its ecological implications. Plenum, New York, 159-175. (1983);
ALLOWAY, B. J.; HARRISON, R. M.; Understanding our envieronment. (2nd edition) Royal Society of Chemistry, Cambrigde; chaper 5. Pg 325-338. (1990);
ALLOWAY, B. J.; HARRISON, R. M., Understanding our environment (3 nd edition) Royal Society of Chemistry, cambridge; chaper 5. Pg 335-400. (1993);
ALLOWAY, B. J. Chapter 5 in Harrison, R. M. (ed) Understanding iur environment (2nd edition), Royal Society of Chemistry, Cambridge (1992);
ALLEN, D. G., DUTY, S. AND WESTERBLAD, H.. Metabolic changes in muscle during exercise; their effects on muscle function. Proceedings of the Australian Physiological and Pharmacological Society 24, 65-75. (1993);
AMANN, R., LEMMER, H., WAGNER, M. Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques. FEMS Microbiologic Ecology 25, 205-215. (1998);
ARAÚJO, E.S. Hidrogeoquímica no entorno de Goiânia (Rios Meia Ponte, João Leite e Santo Antônio): Um estudo comparativo da qualidade da água. Gazeta Tecnológica, informativo do Conselho Regional de Engenharia, Arquitetura e Agronomia. (CREA-GO). Goiânia, v.8, n.1 p.2-8, (2001);
ARPIN, C., C. COZE, A. M. ROGUES, J. P. GACHIE, C. BEBEAR, AND C. QUENTIN.. Epidemiological study of an outbreak due to multidrug-resistant Enterobacter aerogenes in a medical intensive care unit. J. Clin. Microbiol. 34:2163–2169. (1996);
ANDREN, ANDERS; BOBER, THOMAS. Silver in the environment: Transport, Fate, and Effects. North Carolina: SETAC, 154 -161 (2000);
BARCAROLLI, INDIANARA. Mecanismos e Liminares de Toxicidade Aguda do Cobre no Isópodo Eurialino Excirolana armata Dana, 1852 (Isopodacirolanidade): Implicações para o Modelo do Ligante Biológico. Rio Grande: Furg, (2004);
BENITEZ, L.; MARTIN GONZÁLEZ, A.; GILARDI, P.; SOTO, T.; RODRÍGUEZ DE LECEA, J. & GUTIÉRREZ, J.C.; The ciliated protozoa Tetrahymena termophila as a biosensor to detect mycotoxins. Letters in Applied Microbiology 19: 489-491. (1994);
BUNCE, N. J.; enverionmental Chemestry. Winnipeg: Wuerz Publishing (1990).
BAKER, R. A.; Organic Substances and Sediments in Water, Vol. 3, Biological. Boca Raton, FL: 14-22; Lewis, 1991c;
BECK, A. T. An inventory for measuring depression. Archives of General Psychiatry 4, 561–571 (1961);
BECK, A. T., Steer, R. A. and Trexler, L. D. AClohol abuse and eventual suicide. Journal of Studies on AClohol 50, 202–209. (1989);
BERGLUND, M. Suicide in aCloholism. Archives of General Psychiatry 41, 888–891. (1984);
BERRIGAN, M.M. Niveles elevados de estrôncio-90 em niños cerca de reactores nucleares. Newsweek Pathfinder, verano (2003);
BIGAZZI, P.E. Autoimmunity induced by metals. I: Chang, L.W. (Ed.) Toxicology of Metals. MA USA, CRC Press, p 835-852. (1996).
BORGES, K. P.; JESUS, G,J.; ANGELIS, D.F; DALFRES, I.JÁB.; RODRIGUES, M.L.B. Avaliação Bacteriológica das águas do jusante dos rios Atibaia e Jaguarí, Município de Paulínia, SP, BR. In: Congresso de Microbilogia, 21. Foz do Iguaçu: S. B. M. 2001, p.23. BRASIL. Resolução nº 344, do Conselho Nacional do Meio Ambiente , 2001
British Medical Association Hazardous Waste and Human Health, Oxford University Press, Oxford. (1991);
CAIO LEANDRO-ALVES; ANATALIA DA SILVA MONTORO FAGUNDES; ANA PAULA DE MORAIS SANTOS; DANIEL LUCAS MAKINO; ABÍLIO LOPES DE OLIVEIRA-NETO.; Avaliação Ecotoxicológica de pesqueiros localizados no município de serra negra – SP Universidade Estadual de Campinas – Unicamp (tese) (2000)
CAIRNS, J., HART, K.M., HENEBRY, M.S., The effects of a sub-lethal dose of copper sulfate on the colonization rate of freshwater protozoan communities. Amer. Midl. Nat. 104. 93-101. 1980.
CAIRNS, J., PRATT, J.R. The scientific basis of bioassays. Hydrobiologia; 188-189. (1989);
CAMPANILI, M.; Apenas 22% dos Resíduos industriais têm tratamento adequado. O Estado de São Paulo. 02-05-2002. Descoberta a maior área de contaminação de lixo químico do Brasil. Jornal Nacional. 09-04-2002.
CASTILHOS, DANILO D.; GUADAGNIN, CLÍSTENES A.; SILVA, MARCELO D. DA; LEITZKE; VOLNEI W.; FERREIRA, LUIS H..; NUNES, MARIA C. Acúmulo de cromo e seus efeitos na fixação biológica de nitrogênio e absorção de nutrientes em soja UFPEL/FAEM/Depto. de Solos- Campus Universitário (2001)
CASTILHOS, D.D.; COSTA, C.N.; PASSIANOTO, C.C.; LIMA, A.C.R.; LIMA.C.L.R.; MULLER, V. Efeitos da adição de cromo hexavalente no crescimento, nodulação e absorção de nutrientes em soja. Ciência Rural, Santa Maria, v.31, n.6, p. 2001
CASTRO J. M. S. M; CÂMARA V, M. Avaliação do programa de vigilância da qualidade da água para consumo humana em Salvador – BA. Revista Baiana de Saúde Pública, nº. 2, p. 212-226, 2004.
CENTENO JOSÉ A.; FINKELMAN, ROBERT B.; SELENIUS O.; A. BRIAN; FUGE R... Essentials of Medical Geology (impacts of the natural environment on public health). Elsevier Academic press (ed.), 2005.
CHANAL, C., D. SIROT, J. P. ROMASZKO, L. BRET, AND J. SIROT.. Survey of extended spectrum b-lactamases among Enterobacteriaceae. J. Antimicrob. Chemother. 38:127–132.( 1996)
Coudron, P. E., E. S. Moland, and C. C. Sanders.. Occurrence and detection of extended-spectrum b-lactamases in members of the family Enterobacteriaceae at a veterans medical center: seek and you may find. J. Clin. Microbiol. 35:2593–2597.( 1997).
CONAMA - Resolução 020/86 (1986) Diário Oficial da União - Seção I: 1134-1135. 30 de julho de 1986.
CONAMA – Conselho Nacional do Meio Ambiente. Resolução Nº.357, de 17 de março de 2005.
CONAMA, de 25 de março de 2004. Estabelecem diretrizes gerais e os procedimentos mínimos para a avaliação do material a ser dragado em águas 1001 estudos, Goiânia, v. 34, n.11/12, p. 979-1003, nov./dez. 2007. jurisdicionais brasileiras, e dá outras providências. Diário Oficial da União, Brasília, 07 de maio de 2004.
CONNELL, D W., MILLEER, G. J., Chemistry and Ecotoxicology og Pllution; Jon Wiley and Sons, New York. (1984)
CONNELL, D. W. AND MILLER, G. J. Chemistry and Ecotoxicology of Pollution, John Wiley abd Sons, Nex York. (1984);
CURDS, C.R., Cockburn, A., Vandyke, J.M., An experimental study of the role of the ciliated protozoa in the activated sludge process. Wat. Poll. Contr. 67, 312-329. 1968.
DIAS N.; MORTARA, R.A.; LIMA, N.; Morphological and physiological changes in Tetrahymena pyriformis for the in vitro cytotoxicity assessment of Triton X-100. Toxicology in Vitro 17: 357-366. 2003.
DUBE, M.G. AND CULP, J.M. Growth responses of periphyton and chronids exposed to biologically treated kraft pulp mill effluent. Water Sci. Technol. v. 35, p. 339–345,1997.
DRYL, S.; MEHR, K.; Physiological and toxic effects of detergents on Paramecium caudatum. Acta Protozoologica. 15: 501-513. 1976.
ECKENFELDER, W. W. Jr.; Industrial water pollution control. McGraw-Hill Series Resources and Environmental Engineering. 3 edition;(2000).
FAVARETTE, JOSÉ ARNALDO E MERCADANTE, CLARINDA. Biologia – coleção Base. Ed. Moderna.
FENCHEL, T.; HARRISON, P.; The significance of bacterial grazing and mineral cycling for the decomposition of particulate detritus. In The Role of Terrestrial and Aquatic Organisms in Decomposition Processes (Anderson, J. M. & MacFadyen, A., eds). Blackwell, Oxford, 285–299. 1976;
FENCHEL, T., Ecology of Protozoa. Springer-Verlag, Berlin. 1987.
FERGUSTON, J. E.; The Heavy Elements: Envieronmental Impact and Health Effects. Oxford: Pergamon Press. (1990)
FERNANDEZ-LEBORANS, G., NOVILLO, A., Changes in trophic structure of a freshwater protozoan community subjected to cadmium. Ecotoxicology and Environmental Safety 25, 271-279. 1993.
FERNANDEZ-LEBORANS, G., NOVILLO, A. Protozoan communities and contamination of several fluvial systems. Water Environment Research 68: 311-319. 1996.
FIGLIOLIA, A.; BENEDETTI, A.; DELLÁBATE, M.T. et al. Potential chromium bio-availability by lactuca sativa grow on two soils amended with tannery leather residues. Frenesius Environmental Bulletin, Basel, v.1, p.406-410, 1992.
FINKELMAN, ROBERT B.; SELENIUS O.; A. Brian; Centeno José A.; Fuge R... Essentials of Medical Geology (impacts of the natural environment on public health). Elsevier Academic press (ed.), 2005.
FITZGERALD, W. F.; AND CLARKSON, T. W.; Mercury and monomethylmercury: present and future concerns. Enverionmental Health Perspectives 96:pp 159-166; (1991).
FLORIN, L., TSOKOGLOU, A. AND HAPPE, T. (2001). "A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain". J. Biol. Chem. 276: 6125–6132.
FOISSNER, W., BERGER, H., BLATTERER, H., KOHMANN, F.,. Taxonomische und oÈ kologische Revision der Ciliaten des Saprobiensystems. Band IV. Bayerisches Landesamt fuÈ r Wasserwirtschaft, MuÈ nchen, pp. 95±102. 1995;
FOISSNER, W., BERGER, H., BLATTERER, H., KOHMANN, F., Morphology and ecology of the ciliated protozoa used as indicators of water quality (Taxonomische und O¨ kologische Revision der Ciliaten des Saprobiensystems). Band. IV: Gymnostomatida, Loxodes, Suctoria. Bayerische Landesamt fuor Wasserwirstschaft, Mu¨nchen. 1995.
FÖSTNER, U.: Inorganic sediment chemistry and elemental speciation. Sediments: Chemistry and Toxicity of Inplace Pollutants, edited by R. Baudo, J. Giesy, and H. Muntau, p. 61 – 106. Boca Raton, FL: Lewis, 1990.
FÖSTNER U: WITTMAN G: Metal Pollution in the Aquatic Environment. Berlin: Springer-Verlag, 1979.
GAUGLHOFER, J. Environmental aspects of taning with chromium. Journal of the Society of Leather Technologists and Chemists, Cincinnati, v.70, p.11, 1985.
GOYER, R. A.; Lead toxicity: current concerns. Environmental Health Perspectives pp 177-187 (1993)
GOULART MICHAEL DAVE C.;Publicação final: Goulart, M. & Callisto, M.. Bioindicadores de qualidade de água como ferramenta em estudos de impacto ambiental. Revista da FAPAM, ano 2, no 1. 2003
GOLDSTEIN, F. W., Y. PEAN, A. ROSATO, J. GERTNER, L. GUTMANN, and the Vigil’Roc Study Group. 1993. Characterization of ceftriaxone-resistant Enterobacteriaceae: a multicenter study in 26 French hospitals. J. Antimicrob. Chemother. 32:595–603.
GUAIME, S. Laudo comprova contaminação dos moradores de Paulínia. O Estado de São Paulo. 23-08-2001
GUIDO PERSOONE; DANIEL DIVE; Toxicity tests on ciliates—A short review. Ecotoxicology and Environmental Safety. Volume 2, Issue 2, Pages 105-114; 1978
GREBECKI A, KUZNICKI L. Autoprotection in Paramecium caudatum by influencing the chemical properties of its medium. Acta Biol Exp 17; pag 71–107. (1956).
GREENWOOD N. N., EARNSHAW A., Chemistry of the Elements, 2nd ed., Butterworth-Heinemann, Oxford, UK, 1997
GREBECKI A, KUZNICKI L. Autoprotection in Paramecium caudatum by influencing the chemical properties of its medium. Acta Biol Exp 17:71–107. (1956).
HARRISON, R. M.; Polluition: Causes, Effects and control. 2 th ed. Cambridge: Royal Society Chemestry. (1990).
HUNGRIA, M.; VARGAS, M.A.T.; SUHET, A.R. PERES, J.R.R. Fixação biológica do nitrogênio em soja. In: Araujo, R.S.; Hungria, M.(Ed.) Microrganismos de importância agrícola. Brasília: EMBRAPA-SPI, 1994.cap.2, p.9-89.
HUTCHINSON, T. C.; AND MEEMA, K. M.; Lead, Mercury, Cadmium and Arsenic in the Environment. New York, Jonn Wiley and Sons, (1987).
IRATO, P., PICCINNI, E. Effects of cadmium and copper on Astasia longa: metal uptake and glutathione levels. Acta Protozool. 35, 281-285. 1996.
IBRAHIM, G.F. et al. Immunological relationships between Salmonella flagelina and between these and flagellins from other species of Enterobacteriaceae. Med. Microbiol. Immunol., Berlin, v.174, p.87-99, 1985c.
IBRAHIM, G.F.; LYONS, M.J. Detection of Salmonellae in foods with an enzyme immunometric assay. J. Food Prot., Des Moines, v.50, n.1, p.5-61, 1987.
ILIANA ACLOCER1, EIKO ITANO, MARIO AUGUSTO ONO E TEREZA CRISTINA R. M. DE OLIVEIRA1* Produção e purificação de anticorpos policlonais para Salmonella Enteritidis (Enterobacteriaceae) Maringá, v. 24, n. 3, p. 671-679, 2002
KLOPP R. Water management for development of water quality in the Rhur river basin. Schriftenr Ver Wasser Boden Lufthyg, Essen, Germany, 105: 105 – 110, 2000.
KRUUS, P.; DEMMER, M., and McCaw: Chemicals in the Environment. Ch. Vll. Morin Heights, Quebec: Polyscience Publications. (1991)
KROUGH, David. Biologia – O guia natural do Mundo. Ed. Prentice Hall. Mercadante, Clarinda. Biologia Volume Único. Ed. Moderna
LESLIE P. H.; An Analysis of the Data for Some Experiments Carried out by Gause with Populations of the Protozoa, Paramecium Aurelia and Paramecium caudatum. Biometrika .Vol. 44, No. 3/4 pp. 314-327 . (1957). disponivel in vwww. Jstor.org. acessado em 24/09/2010.
LOSI, M.E.; AMRHEIN, C.; FRANKENBER GER, W.T. Environmental biochemistry of chromium. Reviews of Environmental Contamination and Toxicology, New York, v.135, p.91-121, 1994.
LYNN, D.H., GILRON, G.L., 1992. A brief review of approaches using ciliated protists to assess aquatic ecosystem health. Journal of Aquatic Ecosystem Health 1, 263±270.
MADONI, P.; ROMEO, M. G.; Acute toxicity of heavy metals towards freshwater ciliated protists. Environmental Pollution, 141: 1-7. 2006.
MADONI P. The acute toxicity of nickel to freshwater ciliates. Environmental Pollution 109 53-59. 2000.
MADONI P., G. ESTEBAN, G. GORBI, Acute toxicity of cadmium, copper, mercury and zinc to ciliates from activated sludge plants, Bull. Environ. Contam. Toxicol. 49: 900–905. 1992.
MADONI P., DAVOLI D. & CHIERICI E.; Comparative analysis of the activated sludge microfauna in several sewage treatment works. War. Res. 27, 1485-1491. 1993.
MADONI P., A sludge biotic index (SBI) for the evaluation of the biological performance of activated sludge plants based on microfauna analysis, Water Res. 28, 67–75. 1994.
MADONI P., D. DAVOLI, G. GORBI, Acute toxicity of lead, chromium and other heavy metals to ciliates from activated sludge plants. Bull. Environ. Contam. Toxicol. 53, 420–425. 1994.
MADONI P., D. DAVOLI, G. GORBI, L. VESCOVI, Toxic effect of heavy metals on the activated sludge protozoan community. Water Res. 30 135–141. 1996.
MARTÍN-GONZÁLEZ, A.; DÍAZ, S.; BORNIQUEL, S.; GALLEGO, A.; GUTIÉRREZ, J.C. Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban wastewater treatment plants. Research in Microbiology 157: 108-118. 2006;
MARTÍN-GONZÁLEZ, A.; DÍAZ, S.; BORNIQUEL, S.; GALLEGO, A.; GUTIÉRREZ, J. C.
Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban
wastewater treatment plants. Research in Microbiology 157: 108-118, (2006);
MARTÍN-GONZÁLEZ, A.; SILVIA DÍAZ, SARA BORNIQUEL, ANDREA GALLEGO, JUAN CARLOS GUTIÉRREZ, Cytotoxicity and bioaccumulation of heavy metals by ciliated protozoa isolated from urban wastewater treatment plants. Research in Microbiology 157: 108–118. 2006.
MARTIN T.R. AND HOLDICH D.M., The acute lethal toxicity of heavy metals to peracarid crustaceans (with particular reference to freshwater asellids and gammarids), Water Res. 20 (1986), pp. 1137–1147.
MASON, C. F. Christopher Frank. Biology of freshwater pollution. 2. ed. New York: Longman Scientific & Technical, 1991.
MEYNBURG, G.; HOLTZ, C.; GOETZ, D.; Internat. Conf. - Heavy Metals in the Environment, Hamburg, 1995.
MERTZ, W.E. Chromium occurence and function in biological systems. Physiology Reviews, Baltimore, v.49, p.163- 239, 1969.
MINISTÉRIO DO MEIO AMBIENTE; Conselho Nacional do Meio Ambiente – CONAMA. Classificação dos corpos de água e diretrizes ambientais para o seu enquadramento, bem como estabelece as condições e padrões de lançamento de efluentes, e dá outras providências. Disponível em: www.mma.gov.br/port/conama/res/res05/res35705.pdf Acesso em:17 agosto 2008.
MIYOSHI, N.; Kawano, T.; Tanaka, M.; Kadono, T.; Kosaka, T.; Kunimoto, M.; Takahashi, T. & Hosoya, H. Use of Paramecium species in bioassays for environmental risk management: determination of IC50 values for water pollutants. Journal of Health Science 49. 429-435. 2003.
MANAHAN, S. E.: Environmental Chemistry, 5th ed. Boca Raton, FL: Lewis, 1991.
MONTVILLE R., DW SCHAFFNER; Photometric method for differentiation of bacteria of the family Enterobacteriaceae at the level of strain affiliation. Appl Environ Microbiol., pg. 69; cit.: 14; 2003.
MOREL, F. M. M., AND HERING, J. G.: Principles and Applications of Aquatic Chemistry. New York: Wiley, 1993.
MORAL, R.; PEDRENO,N.; GOMEZ, I. et al. Effects of chromium on the nutrient element content and morphology of tomato. Journal of Plant Nutrition,New York, v.18, n.4, p. 815-822, 1995. Langston, W. Toxic effects of metals and the incidence of metal pollution in marine ecosystem. In: Fumess, R.; and Rainbow, H. Ed. Heavy Metals in the Marine Environment. Boca Raton, FL: CRC Press; 102-122. 1990.
MUNG, M. CPI vai pedir interdição de terminal da Shell em SP. O estado de são paulo. 03-05-2002.
MORGAN, G.B., Lackey, J.B. BOD determinations in wastes containing chelated copper and chromium. Sewage Industrial Wastes 30, 283-286. 1958.
NECHAMKIN H., The Chemistry of the Elements, McGraw-Hill, New York, 1968.
NALECZ-JAWECKI, G. Spirotox- Spirostomum ambiguum Acute Toxicity Test- 10 years of experience. Environmental Toxicology 19: 359-364. 2004.
NALECZ-JAWECKI, G. & SAWICKI, J. SPIROTOX- a new tool for testing the toxicity of volatile compounds. Chemosphere 38: 3211-3218. 1999.
NALECZ-JAWECKI, G. & SAWICKI, J. The toxicity of tri-substituted benzenes to the protozoan ciliate Spirostomum ambiguum. Chemosphere 46: 333-337. 2002.
NAŁECZ-JAWECKI G., J. SAWICKI. Toxicity of Inorganic Compounds in the Spirotox Test: A Miniaturized Version of the Spirostomum ambiguum Test. Archive Environmental Contamionation and Toxicology v. 34, pag 1–5 (1998)
NEWMAN, M. C., and McIntosh, A. W. (eds.)? Metal Ecotoxicology. Concepts and Applications. Chelsea, MI? Lewis, 1991.
NICOLAU , A.; DIAS, N.; CARVALHO, G.S.; MOTA, M. & LIMA, N. Os protozoários como ferramenta da monitorização biotecnológica da poluição: ensaios in vitro. 10p. 1999.
NICOLAU, A. MARTINS, M. J; MOTA, M. & LIMA, N. Estudo da comunidade de protozoários exposta a tóxicos em estações de tratamentos de águas residuais. 10p. 1999.
NILSSON, J.R. Tetrahymena in cytotoxicity: with special reference to effects of heavy metals and selected drugs. European Journal of Protistology 25: 2-25. 1989.
NILSSON, J. R.. Tetrahymena in cytotoxicology: With special reference to heavy metals and selected drugs. Eur. J. Protistol. 25; 2- 25. 1989.
NUSCH E. N. Prufung der biologischen Schadwirkungen vonWasserinhaltsstoffen mit Hilfe von Protozoentests. Decheniana Beih v. 26; pag. 87–98. (1982).
ODUM, E.P.; Fundamentals of Ecology, 7ª edição– Fundação Calouste Gulbenkian, Lisboa. 928p. 2004
OGAWA, T.; USUI, M.; YATOME, C. et al. Influence of chromium compounds on microbial growth and nucleic acid synthesis. Bulletin of Environmental Contaminationand Toxicology, v.43, p.254-260, 1989.
PACE, M.L. & ORCUTT, JR J.D. The relative importance of protozoans, rotifers and crustaceans in a freshwater zooplankton community. Limnology and Oceanography 36, 822-830. 1981
PAIVA, E.M.C.D., PAIVA J.B.D., COSTAS M.F.T. E SANTOS F.A . Concentração de sedimentos em suspensão em uma pequena bacia hidrográfica em urbanização. In Congresso Brasileiro de Engenharia Sanitária e Ambiental, 21 João Pessoa – PB, 2001. Anais... João Pessoa: ABES, 2001. CD-ROM.
PARKER G. A., Assessment strategy and the evolution of fighting behaviour. Journal of Theoretical Biology 47 (1974), pp. 223–243.
PARKER G. A., Courtship persistence and female quarding as male time investment strategies. Behaviour 48 (1974), pp. 157–184.
PARKER G. A., Sexual selection and sexual conflict. In: M. S. Blum and N. A. Blum, Editors, Sexual Selection and Reproductive Competition in Insects, Academic Press, New York (1979), pp. 123–166.
PECLZAR, MICHAEL J.;CHAN E. C. S.; NOEL R. KRIEG; Microbiologia: Conceitos e Aplicações. Volume 1, 2ª Ed. 2004.
PIERRE JOLICOUER, ALFRED A. HEUSNER, Biometrics 42. pg 785-794, 1986
PUYTORAC, P.; KATTAR, M.R.; GROLIËRE, C.A. & SILVA-NETO, I.. Polymorphism and ultrastructure of a colpodean ciliate of the genus Platyophrydes Foissner, 1987. Journal of Protozoology, 39: 154- 159. 1992;
RAND, PH. D.; GARY, M.; Fundamentals of aquatic toxicology Effects enverionmental Fate, an Disk Assessemnt 2 th (ed) 1995.
RAINBOW PS, AMIARD-TRIQUET C, AMIARD JC, SMITH BD, LANGSTON WJ. Observations on the interaction of zinc and cadmium uptake rates in crustaceans (amphipods and crabs) from coastal sites in UK and France differentially enriched with trace metals. Aquat Toxicol. 50: 189–204. 2000.
RINO C.A .F., SILVA N.L., HERCULIANI L.A. et al. Avaliação da qualidade das águas dos mananciais da cidade de Lins – SP. : Simpósio Luso-brasileiro de Engenharia Sanitária e Ambiental, 9. Porto Seguro, 2000. Anais... Porto Seguro: SILUBESA/ABES. P.97-105,2000.
ROCHA ODETE, TAVARES L. H. Sipaúba; Cultivo em larga escala de organismos planctônicos para otimização de larvas e alevinos de peixes: II- ORGANISMOS ZOOPLANCTONICOS. biotemas7 (1 e2) pg. 94-109 (1994).
SAUVANT, M.P.; PEPIN, D; BOHATIER, J & GROLIÈRE, C.A. Microplate technique for screening and assessing cytotoxicity of xenobiotics with Tetrahymena pyriformis. Ecotoxicology and Environmental Safety 32: 159-165. 1995.
SAUVANT, M.P.; PEPIN, D; BOHATIER, J & GROLIÈRE, C.A & GUILLOT, J. Toxicity Assessment of 16 Inorganic Environmental Pollutants by Six Bioassays. Ecotoxicology and Environmental Safety 37: 131-140. 1997.
SALVADO´ H, GRACIA MP, AMIGO´ JM. Capability of ciliated protozoa as indicators of environment quality in activated sludge plants. Water Res. 29:1041-1050. 1995
SHERR, B.F., SHERR, E.B., HOPKINSON, C.S. Trophic interactions within pelagic microbial communities: indications of feedback regulation of carbon flow. Hydrobiologia 159, 19-26. 1988.
SASSI, R. & MELO, G.N. Contribuindo para o conhecimento da fauna de protozo·rios do estu·rio do rio ParaÌba do Norte: tintinÌdeos do rio Mandacarÿ. Revista Nordestina de Biologia, 5: 141-155; 1982.
SHERR,E.B. & SHERR, B. F. 1988. Role of microbes in pelagic food webs: a revised concept. Limnology and Oceanography, 33: 1225-1227.
SMITH, S. HESTER R. E. understanding our environment (2 edition) royal societyy of chemestry, Londos ; chaper 5. (1992).
SALGADO, P. E. T. Toxicologia dos metais. In: OGA, S. Fundamentos de toxicologia. São Paulo, 1996. cap. 3.2, p. 154-172.
SALGADO, P. E. T. Metais em alimentos. In: OGA, S. Fundamentos de toxicologia. São Paulo, 1996. cap. 5.2, p. 443-460.
SHUJA, R.N. & SHAKOORI, A.R. Identification and cloning of first cadmium metallothionein like
gene from locally isolated ciliate, Paramecium sp. Molecular Biology Reports 36: 549-560.
(2009);
SIMANOV, A.L., GUSAKOV, A.V., SINITSYN, A.P., IZUMRUDOV, V.A. Synthesis of homopolyribonucleotides using polynucleotide phosphorylase from a thermophilic microorganism in the presence of synthetic polycation. Bioorg. Khimiya, v.20, No.10, pp.1107-1113 (in Russian). (1994);
SMITH, S. (1986) Chapter 5 in Hester, R. E. (ed) Understanding Our Environment (1st edition), Royal Society of Chemistry, London.
SHAKOORI AR, REHMAN A, Haq RU (2004) Multiple metal resistance in he ciliate protozoan, Vorticella microstoma, isolated from ndustrial effluents and its potential in bioremediation of toxic astes. Bull Environ Contam Toxicol 72:1046–1051
SHERR, E.B. & SHERR, B.F. 1994. Bacterivory and herbivory: key roles of phagotrophic protists in pelagic food webs. Microbial Ecology, 28: 223-235.
SLDECEK, V. 1973. System of water quality from the biological point of view. Ergebnisse der Limnologie, 7: 1-218.
STOECK, T. & SCHMIDT, H.J. 1998. Fast and accurate identification of european species of the Paramecium aurelia complex by RAPD-fingerprints. Microbial Ecology, 35:311-317.
STUMM, W., and Morgan, J J.: Aquatic Chemistry, 2nd ed. New York: Wiley-Interscience, 1981.
STUMM, W. (ed.): Aquatic Chemical Kinetics – Reaction Rates of Processes in Natural Waters. New York: Wiley, 1990.
TRUHAUT, R.; Ecotoxicology- A New branch of toxicology: a gemeral survery of its aims, methods, and prospests. Ecology Research edited by ADMc-Intyre, CF. Mills. Pp3-24; New York: Plenum 1975.
TRUHAUT, R.; Ecotoxicology: Objectives principles and perspectives. Ecotoxicol Enverionmental. Safety 1: PP 151-173 (1997)
TORTORA, GERARD J.; FUNKE, BERDELL R.; CASE, CHRISTINE L.; Microbiologia: an Introduction, 8ª ed. 2004.
TRUHALT, R.: Ecotoxicology –a new branch of toxicolog: a general survey of its aims, methods, and prospect. Ecological Toxicology Research, edited by AD McIntyre, CF Mills, pp. 3-24. New York: Plenum, 1975.
TRUHALT, R.: Ecotoxicology: objectives, principles and perspectives. Ecotoxicol Environ. Safety 1:151 – 173, 1977.
TREVORS, J. T.; STRATDON, G. W. & GADD, G. M. Cadmium transport, resistance, and toxicity in bacteria, algae, and fungi. Can. J. Microbiol., 32: 447-460, 1986.
TRUHALt, R.: Ecotoxicology –a new branch of toxicolog: a general survey of its aims, methods, and prospect. Ecological Toxicology Research, edited by AD McIntyre, CF Mills, pp. 3-24. New York: Plenum, 1975.
TRUHALT, R.: Ecotoxicology: objectives, principles and perspectives. Ecotoxicol Environ. Safety 1:151 – 173, 1977.
TURLEY, C.M.; LOCHTE, K. & PATTERSON, D.J. 1988. A barophilic flagellate isolated from 4.500 m in mid-North Atlantic. Deep Sea Research, 35: 1079-1092.
TONANI, KARINA APARECIDA DE ABREU; Identificação e quantificação de metais pesados, parasitas e bactérias em esgoto bruto e tratado da Estação de Tratamento de Esgoto de Ribeirão Preto – SP; (2008/). Disponível em: http://www.teses.usp.br/teses/disponiveis/22/22133/tde-12032008-100717. (Acessado em 24/10/2008).
TWAGILIMANA, L.; BOHATIER, J.; GROLIÈRE, C-A; BONNEMOY, F. & SARGOS, D. A new low cost microbiotest with the protozoan Spirostomum teres: culture conditions and assessment of sensitivity of the ciliate to 14 pure chemicals. Ecotoxicology and Environmental Safety 41: 231-244. 1998.
VASCONCELOS S.M.S; SERAFINI A .B. Ocorrência de indicadores de poluição no Rio Meia Ponte e Ribeirão João Leite – Goiàs e sua relação com Parâmetros de Controle de Qualidade da Água: Coliformes Totais e Fecais. 2002
VELHO,L.F.M.; LANSAC-TÙHA, F.A. & Serafim-Junior, M. 1996. Testate amoebae (Rhizopodea-Sarcodina) from zooplankton of the high Paran· River Floodplain, State Mato Grosso do Sul, Brazil: I. Families Arcellidae and Centropyxidae. Studies on Neotropical Fauna and Environment, 31 (1): 135-150.
VELHO, L.F.M. & LANSAC-TÙHA, F.A. 1996. Testate amoebae (Rhizopodea-Sarcodina) from zooplankton of the high Paran· river floodplain, state of Mato Grosso do Sul, Brazil: II.Family Difflugidae. Studies on Neotropical Fauna and Environment, 31 (3): 174-192.
VENDRAME I.F. & ALVES M.A .S. Qualidade das águas de córregos em São José dos Campos. In: Congresso Brasileiro de Engenharia Sanitária e Ambiental, 21 João Pessoa 2001. Anais... João Pessoa, PB: ABES, 2001. CD-ROM.
VIARENGO A Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level. Critical Rev Aquat Sci 1: 295–317. 1989.
VICKERMAN, K.. The diversity and ecological significance of Protozoa. Biodiversity and Conservation, 1:334-341. 1992.
YOSHIOKA, Y; OSE, Y & SATO, T. Testing for the toxicity of chemicals with Tetrahymena pyriformis. Science of Total Environment 43: 149-157. 1993;
WHITFIELD, M., and Turner, D. R.: The role of particles in regulation of seawater. Aquatic Surface Chemistry, edited by W. Stumm, pp. 457-494. New York: Wiley, 1987.
WALKER, I.. The thecamoebae (Protozoa, Rhizopoda) of small amazonian forest streams and their possible use as indicator organisms for water quality. Suplement Acta Amazonica, 12: 79-105; 1982;
WALKER, I.. On the structure and ecology of the micro-fauna in the central amazonian forest stream ìIgarapÈ-Cachoeiraî. Hydrobiologia,
: 137-152. 1985;
WHITFIELD, M., AND TURNER, D. R.: The role of particles in regulation of seawater. Aquatic Surface Chemistry, edited by W. Stumm, pp. 457-494. New York: Wiley, 1987.
ZAGATTO, P.A.; BERTOLETTI, E. Ecotoxicologia Aquática – princípios e aplicações. Rima Editora, São Carlos, SP. 478 pp. 2006.