Analysis of richness and evenness in the abundance of bacteria that make up the intestinal microbiota of Apis mellifera bees from different biomes in Paraíba

Authors

  • Kilmer Oliveira Soares
  • Thamara Ferreira da Rocha
  • Adryele Gomes Maia
  • Adriana Evangelista Rodrigues
  • Celso José Bruno de Oliveira
  • Rosilene Agra da Silva
  • Aline Carla de Medeiros GVAA - Grupo Verde de Agroecologia e Abelhas http://orcid.org/0000-0002-0161-3541
  • Patricio Borges Maracaja

Keywords:

Microbiota intestinal, Riqueza, Uniformidade

Abstract

The Africanized honey bee (Apis mellifera scutellata x spp.) is a polyhybrid originated from the crossbred between the African subspecies Apis mellifera scutellata and European subspecies and was developed in Brazil in the mid-twentieth century. As in many other beings, the intestine of bees is dominated by a range of microorganisms that perform several important functions for the metabolism, digestion and immunity of their hosts. It is known that the landscape where bees are inserted models the composition and diversity of the intestinal microbiota. A diverse community of bacteria guarantees the diversity of functions as well as the uniformity in the distribution of the abundance of these microorganisms, which is a sign that none or few of them stand out, as this is a sign of dysbiosis. In order to compare the phylogenetic diversity of the intestinal microbiota of A. mellifera bees, 5 samples of 20 individuals were collected from different hives belonging to the Federal University of Paraíba from different regions of the State, Areia (Atlantic Forest) and São João do Cariri (Caatinga), and sent to the Laboratory of Analysis of Products of Animal Origin (LAPOA / CCA / UFPB), in the municipality of Areia - PB. In a sterile environment, all the abdominal contents of the bees were collected and the DNA was extracted. Genomic libraries were prepared and sequencing was performed on a MiSeq equipment (Illumina). The obtained sequences were processed using the DADA2 software. The Shannon richness and Pielou evenness indexes were obtained using the QIIME 2-2020 platform, and the indexes for each region were compared using the Kruskal-Wallis test at 5% probability. The Shannon and Pielou diversity indexes did not differ significantly (Shannon index p-value = 0.754; Pielou index p-value = 0.754) among samples from different biomes in Paraíba. Bees from different biomes showed similar richness, abundance and distribution of abundances.

Author Biography

Aline Carla de Medeiros, GVAA - Grupo Verde de Agroecologia e Abelhas

Possui Licenciatura Plena em Biologia pela Universidade Estadual Vale do Acaraú, UVA/UNAVIDA, concluído em 2008; Curso de Especialização e Educação Ambiental pelas Faculdades Integradas de Patos (FIP), concluído no ano de 2011; Mestrado em Sistemas Agroindustriais, pela Universidade Federal de Campina Grande-UFCG/Pombal-PB, concluído em 2014, é Doutoranda pelo Programa de Pós-Graduação em Engenharia de Processos pela UFCG- Campina Grande/PB. Atua como pesquisadora junto ao CCTA/UFCG/GVAA- Grupo Verde de Agroecologia e Abelha-Pombal-PB (sob orientação dos professores: Prof. D. Sc. Patrício Borges Maracajá e a Prof. D. Sc. Líbia de Sousa Conrado Oliveira). 

Published

2023-09-03

How to Cite

Soares, K. O., Rocha, T. F. da, Maia, A. G., Rodrigues, A. E., Oliveira, C. J. B. de, Silva, R. A. da, Medeiros, A. C. de, & Maracaja, P. B. (2023). Analysis of richness and evenness in the abundance of bacteria that make up the intestinal microbiota of Apis mellifera bees from different biomes in Paraíba. Caderno Verde De Agroecologia E Desenvolvimento Sustentável, 12(1). Retrieved from https://www.gvaa.com.br/revista/index.php/CVADS/article/view/10015

Issue

Section

VI EVENTO TÉCNICO-CIENTÍFICO DO FESTIVAL DO MEL DE SÃO JOSÉ DOS CORDEIROS 2023

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